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Image Search Results
Journal: Cancers
Article Title: miR-214-3p Is Commonly Downregulated by EWS-FLI1 and by CD99 and Its Restoration Limits Ewing Sarcoma Aggressiveness
doi: 10.3390/cancers14071762
Figure Lengend Snippet: Effect of EWS-FLI1 and CD99 silencing on miR-214-3p expression. ( A ) The Venn diagram shows that miR-214-3p was the only common miRNA for EWS-FLI and CD99 from three miRNA data-bases. ( B ) Evaluation of miR-214-3p and EWS-FL1 expression by qRT-PCR in EWS-FLI1-silenced cells, A673/TR/shEF. ( C ) Evaluation of miR-214-3p and in CD99-silenced TC-CD99-shRNA cells (stable model; C, left); CD99 and miR-214-3p expression inA673p6TR/pTERshCD99 cells (inducible model; C, right). ( D ) MiR-214-3p expression analysis by qRT-PCR in a panel of human patient-derived cell lines (LAP-35, A673, IOR-BRZ, IOR-CAR, RD-ES, SK-ES-1, TC-71, SK-N-MC), PDX-derived cell lines (PDX-EW#5-C, PDX-EW#2-C) and human bone-marrow-derived mesenchymal cells (HS196, HS244, HS249, HS128). The data are shown as the mean ± SE of three independent biological experiments (* p < 0.05, ** p < 0.01 Student’s t test).
Article Snippet: EWS human cell line TC-71 cells were kindly provided by T.J. Triche (Children’s Hospital, Los Angeles, CA, USA) and SK-N-MC (ATCC Cat# CRL-2270),
Techniques: Expressing, Quantitative RT-PCR, shRNA, Derivative Assay